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Background: Nardostachys jatamansi DC. is a small, perennial, dwarf, hairy, rhizomatous, herbaceous, endangered and most primitive species within family Valerianacae and is commonly known as ‘Spikenard’ in English. It is mainly distributed in the Himalayas from Pakistan, India (Jammu and Kashmir, Himachal Pradesh, Uttarakhand, Sikkim) to Nepal, Tibet and China. Jatamansi is very famous plant for mental disorders like as insanity, epilepsy etc. and has several activities including anticonvulsant activity, antiparkinson’s activity, tranquillizing activity, hepatoprotective, neuroprotective, hypotensive, anti-diabetic activity. The aim of present article is to put forward the pharmacognostical analysis of rhizome of Nardostachys jatamansi DC. Methods: Macroscopic evaluation, microscopic evaluation, physicochemical evaluation, extractive values, phytochemical analysis, T.L.C. study were carried out using rhizome of Nardostachys jatamansi DC. and data was obtained. Results: Data pertaining to the above cited evaluations was recorded for rhizome of Nardostachys jatamansi DC. Conclusion: All the values hence obtained were subjected to comparison with their corresponding standard values as mentioned in API. It was observed that all the values were under their normal range.