ISOLATION AND CHARACTERISATION OF DEGRADATION PRODUCT OF NORTRIPTILINE
A simple, accurate, precise, sensitive& economic stability indicating HPTLC method was developed and validated for nortriptyline. The method was validated as per ICH guidelines, demonstrating to be accurate and precise with in the corresponding linearity range of titled analytes. Inherent stability of the drug was studied by exposing drugs to various stress conditions as per ICH guidelines namely oxidative, photolysis, acidic, basic and thermal. Chromatographic separation was performed on aluminium plate percoated with silica gel using toluene: ethyl acetate: methanol: glacial acetic acid(4:4:1.6:0.4) as mobile phase. The wavelength selected for scanning was 279nm. Regression plots revealed linear relationship in the conc. Range of 200 to 600 ng/band for CAN and HCT respectively. The correlation coefficient curves was found to be 0.998 for analytes. The proposed method can be used for the routine estimation of nortriptyline in bulk and can be employed for stability indicating analysis.