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Ayurveda is the science of life and aims at maintaining the health of Healthy individuals as well as treating the diseased ones. There are wide range of herbal and mineral drugs are used in Ayurvedic medicines. Rasashastra and Bhaishajya Kalpana is the branch of Ayurveda having mandate of formulation development and its use. There are potent drugs in Ayurveda that are being used for treatment of number of infectious diseases. In modern science the Antibiotics are used for the treatment of infectious diseases. But the Antibiotics has its limitations. In today’s modern life, Antibiotic resistance have become an important threat in developing countries like India, because of significant death rate due to the development of resistance to the existing antimicrobial agents. So for this methods for antimicrobial activity testing can be used for drug discovery. Antibacterial activity of a classical herbo-mineral formulations such as Panchavaktra Rasa (PR), Tribhuvana Kirti Rasa (TKR) were tested against certain pathogens such as Streptococcus pyrogenes, Escherichia coli, Staphyloccous aureus, Pseudomonas aeruginosa, and Salmonella typhi. It was in vitro study of antimicrobial activities in DMSO (Dimethyl Sulphoxide) dissolved samples were tested through well diffusion method and result shows antimicrobial activity in different bacteria were moderate to high in higher concentration.

KEYWORDS: Panchavaktra Rasa, Tribhuvana Kirti Rasa, Herbo-mineral, Antibacterial resistance, DMSO etc.