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The objective of this review study is to analyze simple, accurate, precise and rapid stability indicating HPLC method for simultaneous determination of Lopinavir and Ritonavir in combined dosage forms.
A validated stability indicating reversed phase high-performance liquid chromatographic method was developed for the quantitative determination of two antiviral drugs viz. lopinavir (LPV) and ritonavir (RTV) on Phenomenex – Luna, C18 (250 x 4.6 mm i.d., 5μ) column using mobile phase composition of Buffer: Acetonitrile (55: 45 % V/V) at a flow rate of 1.5 ml/min.
Quantification was achieved with ultraviolet detection at 255nm. The retention time obtained for ritonavir was at 10.92 min and for lopinavir was at 13.23 min. This method has been validated and shown to be specific, sensitive, precise, linear, accurate, rugged, robust and fast.