DEVELOPEMNT AND VALIDATION OF RP-HPLC METH0D FOR SIMULTANEOUS ESTIMATION OF CURCUMIN AND DIOSGENIN FROM POLYHERBAL FORMULATION
Curcumin with Diosgenin reduces inflammations, improves blood sugar levels and reduces stress, anxiety and fatigue. A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for simultaneous estimation of Curcumin and Diosgenin from polyherbal dosage forms. The method was carried out on a Phenomenex C18 column (250 mm *4.5 mm *5µm) with a mobile phase consisting of KH2PO4 buffer (PH adjusted to 3): Acetonitrile (60:40 %v/v) at flow rate of 1ml/min and PDA detection at 210 nm. The retention time of Curcumin and Diosgenin was found to be 2.6 and 4.5 min respectively. The drugs were found to be linear with correlation coefficient which was nearly 0.9992 and 0.9991 for Curcumin and Diosgenin respectively with linearity range of 500 – 1500 µg/ml. Percentage recoveries from recovery studies were found to be 99.33% and 99.61% for curcumin and Diosgenin individually. LOD and LOQ was found to be lower, hence the method is sensitive.