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There is increasing interest in the health and wellness benefits of herbs and botanicals. Natural medicine is a precious resource of therapeutically active components compounds and has increasingly attracted the attention of researchers; further many studies have reported that herbal diuretics might be a useful tool in the treatment of hypertension and chronic kidney diseases. There are a growing number of studies purporting diuretic effects with traditional medicines.
Diuretics drugs increase the rate of urine flow and adjust the volume and composition of body fluids. Drug-induced diuresis is beneficial for the treatment of many maladies such as congestive heart failure (CHF), chronic renal failure, nephritis, cirrhosis, hypertension and pregnancy-induced toxemia. Medicinal herbs are the significant source as Diuretics. Mono and poly-herbal preparations have been used as diuretics. According to one estimate, more than 650 mono and poly-herbal preparations in the form of decoction, tincture, tablets and capsules from more than 75 plants are in clinical use. The various methods for screening of diuretic agents provides useful tool to evaluate the safety and effectiveness of the drugs. It is also useful for determining the dose level of particular class of diuretic agents. The present article gives a brief review about different methods (in-vitro and in-vivo) used for studying diuretic activity.